|
|
Position: Professor
Education:
Ph.D., 1989, University of Colorado at Boulder
Email:
bentley@eng.umd.edu
bentley@umbi.umd.edu
Voice: (301) 405-4321
Homepage: Bentley Lab
Mailing info CBR Faculty Directory
|
Research Overview
Regulation of Gene Expression
The chromosomes of every type of cell carry two major kinds of genetic information: DNA sequences that ultimately specify the amino acid sequence of proteins, and sequences that regulate the expression of those protein-coding sequences. Since DNA is first transcribed into RNA sequences, and then some of these RNA sequences are translated into proteins, cells control the expression of their genes at several levels including the rate at which specificRNA sequences are made, the ways in which those RNA sequences are modified, the rate at which modified RNA sequences are translated into protein chains, and the changes that occur during and after protein chains are synthesized, which includes such changes as...
Complete Information...
|
Research Description
Research Areas: Pathobiology, Biomolecular and Metabolic Engineering, Genome Sciences
Research Specialties: Biomolecular and metabolic engineering; cell-cell communication, heterologous protein expression, insect cell/baculovirus systems; device/bio interfaces
We employ the tools of "functional" genomics to understand the regulation of genetic circuits during applied stresses. In particular, we use DNA microarrays for analyzing gene expression on a global basis. This, coupled with transcriptional promoter probes, quantitative RT-PCR, Northern and Western analyses ultimately enables close to real time detection of gene expression in targeted circuits. We are currently focusing on stress-related and nutritionally- regulated pathways such as those involving s32 , sS , and sN
Our objective for this genomics research is to alter the intracellular environment to improve cellular processes, including the production of recombinant proteins. In order to make use of the vast quantities of data, we need to organize them in reduced dimensional space, develop appropriate mathematical models, then ultimately control phenotypic behavior. This is a component of Systems Biology. One modeling technique currently under investigation is the stochastic Petri net. We are also actively pursuing...
Complete Information...
|
Additional Information
Fischell Department of Bioengineering at the University of Maryland
Elected Fellow, American Association for the Advancement of Science
Elected Fellow, American Institute for Medical and Biological Engineering
|
Representative Publications
Lewandowski, A.T., Small, D.A., Chen, T., Payne, G.F., and W.E. Bentley (2006) Tyrosine-based activatable pro-tag: enzyme-catalyzed protein capture and release, Biotechnology and Bioengineering, 93:1207-1215.
Chang, W., Small, D.A., Toghrol, F., and W.E. Bentley (2006) Global Transcriptome Analysis of Staphylococcus aureus Response to Hydrogen Peroxide, Journal of Bacteriology, 188:1648-1659.
Gonzalez Barrios, A.F., Zuo R., Hashimoto, Y., Yang, L., Bentley W.E., and T.K. Wood (2006) Autoinducer 2 Controls Biofilm Formation in Escherichia coli Through a Novel Motility Quorum-sensing Regulator (MqsR, B3022), Journal of Bacteriology, 188:205-316.
Complete Listing...
|
|
